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murine premeiotic germ cell line gc1 spg  (ATCC)


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    ATCC murine premeiotic germ cell line gc1 spg
    Murine Premeiotic Germ Cell Line Gc1 Spg, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gc1+spg+cell+line/GC-1+spg/pm37956710-151-19-25
    Average 95 stars, based on 232 article reviews
    murine premeiotic germ cell line gc1 spg - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Mrhl Long Noncoding RNA Mediates Meiotic Commitment of Mouse Spermatogonial Cells by Regulating Sox8 Expression
    Article Snippet: Cells of the Gc1-Spg cell line (CRL-2053) and TM4 cell line (CRL-1715) were obtained from ATCC.

    Article Title: Mrhl Long Noncoding RNA Mediates Meiotic Commitment of Mouse Spermatogonial Cells by Regulating Sox8 Expression
    Article Snippet: All chemical reagents were of analytical reagent (AR) grade and were purchased from Sigma.

    Cell Culture:

    Article Title: Genome wide chromatin occupancy of mrhl RNA and its role in gene regulation in mouse spermatogonial cells
    Article Snippet: .. Gc1-Spg cell line (type B spermatogonia) was obtained from the American Type Culture Collection (ATCC) and was cultured in Dulbecco's modified Eagle's medium (Sigma) supplemented with 10% fetal bovine serum (Invitrogen) and 100units/ml penicillin-streptomycin solution (Sigma) at 37 o C in a humidified 5% CO 2 atmosphere. ..

    Article Title: Connective tissue growth factor mediates mouse spermatogonial migration associated with differentiation.
    Article Snippet: The undifferentiated spermatogonial cell line C18-4 was a kind gift from Dr. Simon Wing (McGill University), and cultured in DMEM (Gibco) supplemented with 5% FBS (Gibco), 2 mM L-glutamine (Invitrogen) and 100 U/mL P/S (Gibco). .. The GC1-spg cell line was purchased from ATCC (CRL-2053; Manassas, VA, USA) and was cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco). ..

    Modification:

    Article Title: Genome wide chromatin occupancy of mrhl RNA and its role in gene regulation in mouse spermatogonial cells
    Article Snippet: .. Gc1-Spg cell line (type B spermatogonia) was obtained from the American Type Culture Collection (ATCC) and was cultured in Dulbecco's modified Eagle's medium (Sigma) supplemented with 10% fetal bovine serum (Invitrogen) and 100units/ml penicillin-streptomycin solution (Sigma) at 37 o C in a humidified 5% CO 2 atmosphere. ..

    Article Title: Expression of Cnnm1 and Its Association with Stemness, Cell Cycle, and Differentiation in Spermatogenic Cells in Mouse Testis.
    Article Snippet: .. pET100 D, puromycin, lipofectamine 2000, Topo TA cloning kit, OptiMEM, goat anti-rabbit IgG-conjugated with Alexa Fluor 488, goat anti-rat IgG-conjugated with Alexa Fluor 488, Rabbit anti-goat IgG-conjugated with Alexa Fluor 568 (Invitrogen); pEGFP-N1 (Clonetech); pBRITCTAP HIS-FLAG (Addgene); trypsin-EDTA, Dulbecco’s Modified Eagle Medium (DMEM), foetal bovine serum (FBS) and GC1-spg cell line (ATCC); Taq DNA polymerase, XhoI, EcoRI and T4 DNA ligase (New England Biolabs); deoxy nucleotide triphosphates (dNTPs), gel purification kit (GE Healthcare); Ready-To-Go T-Prime first strand synthesis kit (Amersham Biosciences); Big Dye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) and FirstChoice RLM-RACE kit (Ambion Inc) were used. .. Antibodies to CNNM1 (sc-68646), OCT 3/4 (sc-365509) and c-KIT (sc-19619) were purchased from Santa Cruz Biotechnology.

    TA Cloning:

    Article Title: Expression of Cnnm1 and Its Association with Stemness, Cell Cycle, and Differentiation in Spermatogenic Cells in Mouse Testis.
    Article Snippet: .. pET100 D, puromycin, lipofectamine 2000, Topo TA cloning kit, OptiMEM, goat anti-rabbit IgG-conjugated with Alexa Fluor 488, goat anti-rat IgG-conjugated with Alexa Fluor 488, Rabbit anti-goat IgG-conjugated with Alexa Fluor 568 (Invitrogen); pEGFP-N1 (Clonetech); pBRITCTAP HIS-FLAG (Addgene); trypsin-EDTA, Dulbecco’s Modified Eagle Medium (DMEM), foetal bovine serum (FBS) and GC1-spg cell line (ATCC); Taq DNA polymerase, XhoI, EcoRI and T4 DNA ligase (New England Biolabs); deoxy nucleotide triphosphates (dNTPs), gel purification kit (GE Healthcare); Ready-To-Go T-Prime first strand synthesis kit (Amersham Biosciences); Big Dye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) and FirstChoice RLM-RACE kit (Ambion Inc) were used. .. Antibodies to CNNM1 (sc-68646), OCT 3/4 (sc-365509) and c-KIT (sc-19619) were purchased from Santa Cruz Biotechnology.

    Gel Purification:

    Article Title: Expression of Cnnm1 and Its Association with Stemness, Cell Cycle, and Differentiation in Spermatogenic Cells in Mouse Testis.
    Article Snippet: .. pET100 D, puromycin, lipofectamine 2000, Topo TA cloning kit, OptiMEM, goat anti-rabbit IgG-conjugated with Alexa Fluor 488, goat anti-rat IgG-conjugated with Alexa Fluor 488, Rabbit anti-goat IgG-conjugated with Alexa Fluor 568 (Invitrogen); pEGFP-N1 (Clonetech); pBRITCTAP HIS-FLAG (Addgene); trypsin-EDTA, Dulbecco’s Modified Eagle Medium (DMEM), foetal bovine serum (FBS) and GC1-spg cell line (ATCC); Taq DNA polymerase, XhoI, EcoRI and T4 DNA ligase (New England Biolabs); deoxy nucleotide triphosphates (dNTPs), gel purification kit (GE Healthcare); Ready-To-Go T-Prime first strand synthesis kit (Amersham Biosciences); Big Dye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) and FirstChoice RLM-RACE kit (Ambion Inc) were used. .. Antibodies to CNNM1 (sc-68646), OCT 3/4 (sc-365509) and c-KIT (sc-19619) were purchased from Santa Cruz Biotechnology.

    Sequencing:

    Article Title: Expression of Cnnm1 and Its Association with Stemness, Cell Cycle, and Differentiation in Spermatogenic Cells in Mouse Testis.
    Article Snippet: .. pET100 D, puromycin, lipofectamine 2000, Topo TA cloning kit, OptiMEM, goat anti-rabbit IgG-conjugated with Alexa Fluor 488, goat anti-rat IgG-conjugated with Alexa Fluor 488, Rabbit anti-goat IgG-conjugated with Alexa Fluor 568 (Invitrogen); pEGFP-N1 (Clonetech); pBRITCTAP HIS-FLAG (Addgene); trypsin-EDTA, Dulbecco’s Modified Eagle Medium (DMEM), foetal bovine serum (FBS) and GC1-spg cell line (ATCC); Taq DNA polymerase, XhoI, EcoRI and T4 DNA ligase (New England Biolabs); deoxy nucleotide triphosphates (dNTPs), gel purification kit (GE Healthcare); Ready-To-Go T-Prime first strand synthesis kit (Amersham Biosciences); Big Dye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) and FirstChoice RLM-RACE kit (Ambion Inc) were used. .. Antibodies to CNNM1 (sc-68646), OCT 3/4 (sc-365509) and c-KIT (sc-19619) were purchased from Santa Cruz Biotechnology.



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